Group Leader/s
Head of lab
intro
The main interest of our laboratory is the search for new tumoral biomarkers for the molecular diagnostics and the characterization of new therapeutic targets, the study of the molecular mechanisms involved in the epithelial-mesenchyma transition, tumoral progression and metastasis in colorectal cancer. Our strategy is based on the application of advanced proteomic tools, such as two-dimensional electrophoresis in combination with DIGE, mass-spectrometry and the use of protein microarrays, to the study of the alterations in proteins and antibodies that occur in plasma samples, tissues and/or tumoral cell lines.
Members
| José Ignacio Casal Álvarez |
| Laura Pintado Berninches |
| Ruben Alvaro Bartolome Conde |
| Angela Martin Regalado |
| Marta Jaen Castaño |
| Javier Robles Sebastian |
Selected Publications
Bartolomé, R.A., Jaén, M., Casal, J.I. [2018]. An IL13Rα2 peptide exhibits therapeutic activity against metastatic colorectal cancer. British Journal of Cancer. 119:940-949.
Casal JI and Bartolomé RA [2018]. RGD cadherins and α2β1 integrin in cancer metastasis: A dangerous liaison. BBA Reviews on Cancer. 1869, 321-332
Bartolome, R.A., Aizpurua, C., Jaen, M., Torres, S., Calviño, E., Imbaud, J.I., Ignacio Casal, J. [2018]. Monoclonal antibodies directed against cadherin RGD exhibit therapeutic activity against melanoma and colorectal cancer metastasis. Clinical Cancer Research. 24:433-444.
Bartolomé RA, S Torres, S Isern de Val, B Escudero-Paniagua, E Calviño, J Teixidó and JI Casal [2017]. VE-cadherin promotes metastasis and consitutes a potential therapeutic target in melanoma and breast cancer. Oncotarget. 8, 215-227
Torres, S., Garcia-Palmero, I., Bartolomé, R.A., Fernandez-Aceñero, M.J., Molina, E., Calviño, E., Segura, M.F., Casal, J.I. [2017]. Combined miRNA profiling and proteomics demonstrates that different miRNAs target a common set of proteins to promote colorectal cancer metastasis. Journal of Pathology. 242:39-51.
García-Palmero I, S Torres, RA Bartolomé, A Peláez-García, MJ Larriba, M Lopez-Lucendo, C Peña, B Escudero-Paniagua, A Muñoz and JI Casal [2016]. Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI) . Oncogene. 40, 5224-36. doi: 10.1038/onc.2016.57
Torres S, I Garcia-Palmero, M Herrera, RA Bartolomé, MJ Fernandez-Aceñero, G Padilla, A Peláez-García, C Peña, M Lopez-Lucendo, A Garcia de Herreros, F Bonilla, JI Casal [2015]. LOXL2 is highly expressed in cancer-associated fibroblasts and associates to poor colorectal cancer outcome. Clin Cancer Res. 21, 4892-902
Bartolomé Rubén A., Irene García-Palmero, Sofía Torres, María López-Lucendo, Irina V. Balyasnikova and Casal JI [2015]. IL-13 receptor α2 signaling requires a scaffold protein, FAM120A, to activate FAK and PI3K pathways in colon cancer metastasis. Cancer Res. 75(12) 2434-44
Torres S, RA Bartolomé, M Mendes, R Barderas, MJ Fernandez-Aceñero, A Peláez-García, C Peña, M Lopez-Lucendo, R Villar-Vázquez, A Garcia de Herreros, F Bonilla, Casal JI [2013]. Proteome profiling of cancer-associated fibroblasts identifies novel proinflammatory signatures and prognostic markers for colorectal cancer. Clin Cancer Res. doi: 10.1158/1078-0432.CCR-13-1130
Bartolomé RA., Barderas R, Torres S, Fernandez-Aceñero MJ, Mendes M, García-Foncillas J, Lopez-Lucendo M, Casal JI [2013]. Cadherin-17 interacts with α2β1 integrin to regulate cell proliferation and adhesion in colorectal cancer cells causing liver metastasis. Oncogene. doi:10.1038/onc.2013.117
Barderas R, Mendes M, Torres S, Bartolome RA, Lopez-Lucendo M, Villar-Vazquez R, Pelaez A, Fuente E, Bonilla F, Casal JI [2013]. In-depth characterization of the secretome of colorectal cancer metastatic cells identifies key proteins in cell adhesion, migration and invasion. Mol Cell Proteomics (doi: 10.1074/mcp.M112.022848)
Barderas R, Bartolomé RA, Fernandez-Aceñero MJ, Torres S, Casal JI [2012]. Interleukin 13 Receptor α2 plays a major role in cell adhesion, migration and invasion in metastatic colorectal cancer. Cancer Res (DOI:10.1158/0008-5472.CAN-11-4090)
Babel, I., Barderas R., Diaz-Uriarte, R., Martínez-Torrecuadrada JL, Sánchez-Carbayo M and JI Casal [2009]. Identification of tumour-associated autoantigens for the diagnosis of colorectal cancer in serum using high-density protein microarrays. Mol Cell Proteomics 8, 2382-2395
R Barderas, J. Desmet, P Timmerman, R Meloen and J. I Casal [2008]. Affinity maturation of antibodies assisted by in silico modelling. PNAS. 105, 9029
Alfonso P., Núñez A., Madoz-Gurpide, J., Lombardia, L., Sánchez, L. and J.I. Casal [2005]. Proteomic expression analysis of colorectal cancer by two dimensional differential gel electrophoresis. Proteomics. 5, 2602
Fellowships and jobs
Predoctoral Fellowship
Buscamos licenciados en Bioquímica/ Biotecnología o titulación relacionada para solicitar becas FPI con objeto de realizar Tesis Doctoral en el laboratorio de Proteóm
Laboratory
CIB Contact
Employment Opportunity
El Laboratorio de Proteómica Funcional del Centro de Investigaciones Biológicas (Madrid) del Consejo Superior de Investigaciones Científicas (CSIC) dirigido por el Dr Ignacio Casal ofrece:
Laboratory
CIB Contact