Our laboratory uses advanced quantitative proteomic differential analysis (SILAC) to study and characterize the proteome of the subcellular compartment of two cell lines KM12C and KM12SM, differing in metastatic properties. Functionality studies of the targeted genes are accomplished by silencing with RNA interference and overexpression studies in tumoral cells. Altogether, these studies constitute a significant step forward in the knowledge and characterization of the proteome of colorectal cancer and the elucidation of the molecular mechanisms underlying CRC. The expected outcome of our studies will consist in the development of panels of biomarkers to facilitate early detection of cancer, cancer recurrences and identification of key players in metastasis progression.