Improving methods for the production of therapeutic molecules
We are interested in the development and improvement of new technologies and production tools for complex protein biologics using yeast expression methodologies and other eukaryotic expression systems. Our group was a member of the FP7 Project ComplexINC, which conceptualised and systematically generated advanced toolkits to enable high-throughput assembly of complex biologics and metabolic pathways using eukaryotic expression systems. The ultimate goal of these toolkits, including two yeast-based toolkits developed in our laboratory, was enabling micro- and large-scale production of high-quality protein biologics for drug discovery and as biotherapeutics.
One of the outreach activities of the ComplexINC project consisted in the edition of a new book on advanced methodologies for protein complex production using a variety of expression hosts and systems, which was published by Springer in spring of 2016. The volume, entitled Advanced Technologies for Protein Complex Production and Characterization (doi:10.1007/978-3-319-27216-0), belongs in the Advances in Experimental Medicine and Biology (v. 896).