Group Leader/s

 

intro

The Environmental Biotechnology group was created to investigate the metabolizing abilities of bacteria for environmental applications. We study the genes, enzymes and regulation of the metabolic pathways both to degrade the environmental contaminants and to synthesize chemical compounds by biotransformation/biorefinery processes (e.g., pharmaceuticals, biofuels, biomaterials, enzymes).

We work on the genetic and biochemical characterization of the bacterial catabolic pathways involved in steroid degradation (catabolic genes and regulators) using Mycobacterium smegmatis as model organism. The knowledge of these pathways allows us to create genetically modified bacteria for the production of steroids of pharmaceutical interest from natural substances even using other organisms as cell factories such as Corynebacterium glutamicum. In the same pharmaceutical field we are developing projects that involve the use of metagenomics techniques for bacterial production of antitumor polyketides with the aim of defining a biotechnological process to ensure its supply to study their mechanisms of action and its future commercialization. In the field of biorefineries we have leaded the European project Synpol (FP7) which aims to develop a platform for biopolymer production by the bacterial fermentation (e.g., Clostridium, Rhodospirillum) of synthesis gas (syngas) generated by pyrolysis of highly complex biological wastes (e.g., municipal, commercial, sludge, agricultural). The group is also working with cyanobacteria to explore their applications in the food sector using Arthrospira (Spirulina) as a model system (INSPIRA1 and A4HW projects) or as novel enviromental solutions using Synechoccocus (LIAR project). Green and Sustainable Chemistry is one of the group’s activities aimed at the development of clean industrial bioprocesses with several applications: i) Oil biodesulfurization to reduce, by using recombinant microorganisms (e.g., Rhodococcus, Pseudomonas), the content in organic sulfur of fossil fuels, ii) Bioenergetics to develop modified microorganisms by metabolic engineering (e.g., Clostridium, Escherichia, Klebsiella, Pseudomonas, Corynebacterium) to produce biofuels (e.g., butanol, isobutanol, 1,3-propanediol); iii) Biocatalytic to produce genetically engineered enzymes with new catalytic properties (e.g., penicillin acylases) or to modify them in order to favor its stability by directed multipoint immobilization processes.

 

SANZ D, GARCÍA JL, DÍAZ E.  [2020]. Expanding the current knowledge and biotechnological applications of the oxygen-independent ortho-phthalate degradation pathway. Environ Microbiol. 22(8):3478-3493.

ALIOTO T, ALEXIOU KG, BARDIL A, BARTERI F, CASTANERA R, CRUZ F, DHINGRA A, DUVAL H, FERNÁNDEZ I MARTÍ Á, FRIAS L, GALÁN B, GARCÍA JL, HOWAD W, GÓMEZ-GARRIDO J, GUT M, JULCA I, MORATA J, PUIGDOMÈNECH P, RIBECA P, RUBIO CABETAS MJ, VLASOVA A, WIRTHENSOHN M, GARCIA-MAS J, GABALDÓN T, CASACUBERTA JM, ARÚS P.  [2020]. Transposons played a major role in the diversification between the closely related almond and peach genomes: results from the almond genome sequence. Plant J. 101(2):455-472.

ZAGHETTO DEALMEIDA, P, BRITO DEOLIVEIR, T., COUTINHO DELUCASA, R., SANTOS SALGADOC, J.C., MARTÍNEZ PÉREZ, M., GÁLAN, B., GARCÍA, J.L., TEIXEIRA DE MORAESPOLIZELIA, M L.  [2020]. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 β-glucosidase from Anoxybacillus thermarum. Proc. Biochem. 99:1-8.

TIMMIS K, CAVICCHIOLI R, GARCIA JL, NOGALES B, CHAVARRÍA M, STEIN L, MCGENITY TJ, WEBSTER N, SINGH BK, HANDELSMAN J, DE LORENZO V, PRUZZO C, TIMMIS J, MARTÍN JLR, VERSTRAETE W, JETTEN M, DANCHIN A, HUANG W, GILBERT J, LAL R, SANTOS H, LEE SY, SESSITSCH A, BONFANTE P, GRAM L, LIN RTP, RON E, KARAHAN ZC, VAN DER MEER JR, ARTUNKAL S, JAHN D, HARPER L.  [2019]. The urgent need for microbiology literacy in society. Environ Microbiol. 21(5):1513-1528

IBERO J, GALÁN B, DÍAZ E, GARCÍA JL.  [2019]. Testosterone Degradative Pathway of Novosphingobium tardaugens. Genes (Basel) 10(11). pii: E871.

DE SOUSA M, MELO VMM, HISSA DC, MANZO RM, MAMMARELLA EJ, ANTUNES ASLM, GARCÍA JL, PESSELA BC, GONÇALVES LRB.  [2019]. One-Step Immobilization and Stabilization of a Recombinant Enterococcus faecium DBFIQ E36 L-Arabinose Isomerase for D-Tagatose Synthesis. Appl Biochem Biotechnol. 188:310-325.

FELPETO-SANTERO C, GALÁN B, LUENGO JM, FERNÁNDEZ-CAÑON JM, DEL CERRO C, MEDRANO FJ, GARCÍA JL.  [2019]. Identification and expression of the 11β-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum. Microb Biotechnol 12(5):856-868.

ULUŞEKER C, TORRES-BACETE J, GARCÍA JL, HANCZYC MM, NOGALES J, KAHRAMANOĞULLARI O.  [2019]. Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels. Sci Rep. 14;9(1):2076.

KAČAR D, SCHLEISSNER C, CAÑEDO LM, RODRÍGUEZ P, DE LA CALLE F, GALÁN B, GARCÍA JL  [2019].  Genome of Labrenzia sp. PHM005 Reveals a Complete and Active Trans-AT PKS Gene Cluster for the Biosynthesis of Labrenzin. Front Microbiol. 10:2561

IBERO J, SANZ D, GALÁN B, DÍAZ E, GARCÍA JL. (2019)  [2019]. High-Quality Whole-Genome Sequence of an Estradiol-Degrading Strain, Novosphingobium tardaugens NBRC 16725. Microbiol Resour Announc. 14;8(11). pii: e01715-18.

 

Funding

CAM, Contrato Programa Grupos Estratégicos (2000–2004)

Fundación Ramón Areces, FRA-XICN-BT (2000-2003)

Repsol YPF (2000-2001)

EU, QLK3-2000-170 (2000-2003)

CICYT, BIO2000-1076 (2000-2003)

CICYT, BCM2000-0125-CO4-02 (2001-2003)

CICYT, BMC2000-1002 (2001-2003)

CICYT, BIO2000-0009-P4-04 (2002-2004) 

CICYT, BIO2000-0060-P4-03 (2002-2004) 

CICYT, GEN2001-4698-C05-02 (2003-2005) 

CAM, 07M/0076/2002 (2003-2004)

CICYT, BIO2003-01482 (2004-2006)

CICYT, VEM2003-20075-C02-02 (2004-2006)

CICYT, BIO2003-05309-C04-02 (2004-2006)

CAM, AMB-259-0505 (2006-2010)

CSIC 2004 2 0E 073

CICYT, BIO2006-05957 (2006-2009)

CICYT, CTM2006-04007 (2006-2007)

CSIC, 2006 2 0I 069 (2006-2007)

CICYT, BFU2006-15214-C03-01 (2006-2009)

CICYT, MMA 039/2006/3-11.2 (2006-2008)

CSIC, 20042 0E 246

CSIC 200520F0193

EU, NMP2-CT-2007-026515 (2006-2010)

CICYT Consolider CSD2007-00005 (2008-2012)

CICYT BIO2007-29812-E (2008-2010)

MICINN, BFU2009-11545-C03-03 (2010-2012)

Biopolis S.L. PLAN E-MICINN (2010-2011)

Biopolis S.L. CENIT-MICINN (2010-2012)

Fundación General del CSIC (2011-2013)

MINECO, INNPACTO IPT-2011-0752-900000 (2011-2014)

MINECO, INNPACTO IPT-2011-1337-010000 (2011-2014)

ARAMCO (2012-2014)

EU, KBBE Call 6-311815 (2012-2016)

MINECO, BIO2012-39695-C02-01 (2013-2015)

MINECO, RTC-2014-1764-3 (2014-2016)

MINECO, RTC-2014-2249-1 (2014-2017)

MINECO, BIO2013-49667-EXP (2014-2016)

Fundación Ramón Areces. XVII Concurso Nacional (2015-2017)

CAM, S2013/ABI-2783 (2014-2018)

EU, FET-OPEN 686585. H2020 (2016-2019)

MINECO, BIO2015-66960-C3-3-R (2016-2019)

- MINECO/FEDER DESPOL RTC-2016-4892-1

- MINECO/FEDER A4HW RTC-2016-4860-2

 

 

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