Group Leader/s



The complex control of terminal cell differentiation to produce gameta is mediated by mechanisms of gene expression regulation interacting with multiple endocrine, paracrine and autocrine factors.

Our laboratory focuses on the study of molecular mechanisms that, trough differential gene expression of both mRNAs and sRNAs (small RNAs), controling the development of oocytes, spermatozoa and early postzygotic stages.

In mammals, differentiation of germ cells starts in the fetal period, progressing in various stages of postnatal life. This requires a high level of molecular and cellular coordination, regulated by differential gene expression. Our group studies the role and regulation of genes involved in gametogenesis in both sexes and their contribution to zygotic preimplantation development and how their deregulation can be potentially responsible for impaired fertility. In this sense, one of our current interest is to identify mechanisms of action, at genetic, epigenetic and cellular level, potentially caused by environmental contaminants compounds able to induce reproductive disorders. Using mouse as experimental model and various approaches such as: high-density DNA microarrays, deep sequencing of RNAs, cell analysis or null mutant mouse models for specific genes, we are studying functional gene regulatory expression and genetic networks during development of different gametogenic cell types from primordial germ cells (PGCs) to mature gametes. In addition to the normal patterns of gene expression, we are studying the effects of various compounds considered as reprotoxicants in relation to their level or period of exposure during development, both during the gametogenesis of individuals directly exposed as possible transgenerational effects associated with epigenetic mechanisms.

Our group is particularly focussed on the study of biogenesis and functional role of regulatory noncoding small RNAs, specifically microRNAs endo-siRNAs and piRNAs ( previously defined as germline specific) and their action as post-transcriptional regulators, both in gametogenesis and in preimplantation zygotes.



Staff Scientists
Jesús del Mazo Martínez
Predoctoral Students
Daniel Pérez Fernández
Team image

Schrenk D, Bignami M, Bodin L, Chipman JK, del Mazo J, Grasl-Kraupp B, Hogstrand C, Hoogenboom LR, Leblanc J-C, Nebbia CS, Ntzani E, Petersen A, Sand S, Schwerdtle T, Vleminckx C, Wallace H, Guerin T, Massanyi P, Van Loveren H, Baert K, Gergelova P and Nielsen E  [2020]. Scientific Opinion on the update of the risk assessment of nickel in food and drinking water. EFSA Journal 2020;18(11):6268, 101 pp. https://doi. org/10.2903/j.efsa.2020.6268

J.del Mazo.  [2020]. La contaminación silenciosa que nos está robando la fertilidad”. The Conversation (ISSN 2201-5639) 26 Feb 2020

Gonzalez-Sanz S., Barreñada O., Rial E., Brieño-Enriquez M., del Mazo J.  [2020]. The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries” Sicentific Reports (2020) 10:18036 |

Schrenk D, Bignami M, Bodin L, Chipman JK, del Mazo J, Hogstrand C, Hoogenboom LR, Leblanc J-C, Nebbia CS, Nielsen E, Ntzani E, Petersen A, Sand S, Schwerdtle T, Vleminckx C, Wallace H, Brimer L, Cottrill B, Dusemund B, Mulder P, Vollmer G, Binaglia M, Ramos Bordajandi L, Riolo F, Rold an-Torres R and Grasl- Kraupp B,.  [2020]. Scientific Opinion – Risk assessment of glycoalkaloids in feed and food, in particular in potatoes and potato-derived products. EFSA Journal 2020;18(8):6222, 190 pp.

Odei Barreñada , Daniel Fernández-Pérez , Eduardo Larriba , Miguel Brieño-Enriquez & Jesús del Mazo  [2020]. Diversification of piRNAs expressed in PGCs and somatic cells during embryonic gonadal development. RNA Biology 2020; 17:1309-23

Schrenk D., Bodin L., Chipman JK., del Mazo J., Grasl-Kraupp B., Hogstrand C., Hoogenboom LR., Jean-Charles Leblanc J-C.,Nebbia CS., Nielsen E., Ntzani E., Petersen A., Sand S., Schwerdtle T., Vleminckx C., Wallace H., Alexander J., Cottrill B., Dusemund B., Mulder P., Arcella D., Baert K., Cascio C., Steinkellner H. and Bignami M.  [2019]. Scientific opinion on the risks for animal and human health related to the presence of quinolizidine alkaloids in feed and food, in particular in lupins and lupin. EFSA Journal (2019);17(11):5860 112pp.



“Reprogramming of DNA methylation during mammalian development and environmental impact of endocrine disruptors”. Grant Agency: CEFIC-LRI. CEFIC (European Chemical Industry Council) Long-range Research Inititative [joining chemical industry associations ACC USA), JCIA (Japan) and CEFIC (Europe)].

“Análisis funcional de microRNAs (miRNAs) en embriogénesis temprana de mamíferos” FIS (MICIN) (PI071007).

"Identification of genetic biomarkers of transgenerational effects of Endocrine Disruptor compounds on the mouse germ cells development”CSIC-ASCR (Czech Republic) (2008CZ0014).

“Análisis funcional de microRNAs en gametogénesis y estadios postcigóticos preimplantacionales”. CSIC (201020E016) 2010-2012.

"Impact of Endocrine Disruptors on genomic DNA methylation patterns in the germline”.Programme National de Recherche sur les Perturbateurs Endocriniens” Ministère de l’Ecologie, du Developpment Durable, des Transports et du Logement. República Francesa.(11-MRES-PNRPE-9-CVS-072-Nº210064934).2012-2014

“Efectos transgeneracionales de disruptores endocrinos ambientales mediados por RNAs pequeños no-codificantes sobre células germinales primordiales” MINECO (BFU2013-42164-R). 2014-2017


More info

  • In March 2009 the European Commission appointed Jesús del Mazo as member of the "Scientific Advisors on Risk Assessment and Public Health" (Commission Decision 2008/721/EC).
  • Media Releases:
  1. - Exposure to Endocrine Disruptor Induces Transgenerational Epigenetic Deregulation of MicroRNAs in Primordial Germ Cells.-
  2. The effects of different endocrine disruptors defining compound-specific alterations of gene expression profiles in the developing testis.