Aspergillus nidulans is a genetic model for studying polarised cell growth and long-distance transport. Hyphal tip cells grow exclusively by apical extension, leading to tubular multinucleated cells where intracellular traffic is tailored to the large distances that exist between apical and distal regions of the cytosol, or between the different nuclei of the cell. We use a combination of formal Genetics with Molecular and Cellular Biology to understand intracellular traffic, including the mechanisms by which environmental signals are transduced to the different nuclei of the cell to ensure coordinated transcriptional responses. The research unit includes two groups, led by Dr. Eduardo A. Espeso and Dr. Miguel A. Peñalva (acting as coordinator), respectively.
Hernández-Gonzalez, M., Bravo-Plaza, I., Pinar, M., de los Ríos, V., Arst, H. N., Jr., and Peñalva, . Endocytic recycling via the TGN underlies the polarized hyphal mode of life. M. A. PLoS Genetics 14: e1007291.
Hernández-González, M., Pantazopoulou, A., Spanoudakis, D., Seegers, C. L. C., and Peñalva, M. A. . Genetic dissection of the secretory route followed by a fungal extracellular glycosyl hydrolase. Mol Microbiol 109: 781-800.
Pantazopoulou, A., Galmarini, C. M., and Peñalva, M. A. . Molecular basis of resistance to the microtubule-depolymerizing antitumor compound plocabulin. Sci Rep 8: 8616
Peñalva, M.A., Zhang, J., Xiang, X., and Pantazopoulou, A. . Transport of fungal RAB11 secretory vesicles involves myosin-5, dynein/dynactin/p25 and kinesin-1 and is independent of kinesin-3. Mol Biol Cell 28, 947-961.
Pinar, M., and Peñalva, M.A. . Aspergillus nidulans BapH is a RAB11 effector that connects membranes in the Spitzenkörper with basal autophagy. Mol Microbiol 106: 452-468.
Pinar, M., Arst, H. N., Jr., Pantazopoulou, A., Tagua, V. G., de los Ríos, V., Rodríguez-Salarichs, J., Díaz, J. F., and Peñalva, M. A. . TRAPPII regulates exocytic Golgi exit by mediating nucleotide exchange on the Ypt31 orthologue RabE/RAB11. Proc Natl Acad Sci U.S.A. 112: 4346-435
Mellado, L., Arst, H.N., Jr., Espeso, E.A. . Proteolytic activation of both components of the cation stress-responsive Slt pathway in Aspergillus nidulans. Molecular Biology of the Cell. 27:2598-2612.
Etxebeste, O., Espeso, E.A. . Neurons show the path: Tip-to-nucleus communication in filamentous fungal development and pathogenesis. FEMS Microbiology Reviews. 40:610-624.
Hernández-Ortiz, P., Espeso, E.A. . Spatiotemporal dynamics of the calcineurin target CrzA. Cellular Signalling. 29:168-180.
Dubey, A.K., Barad, S., Luria, N., Kumar, D., Espeso, E.A., Prusky, D.B. . Cation-stress-responsive transcription factors SltA and CrzA regulate morphogenetic processes and pathogenicity of colletotrichum gloeosporioides. PLoS ONE. 11:-.
Intracellular Membrane Trafficking Group, PI: Miguel A. Peñalva
Funding body: DGICYT (Dirección General de Investigación Científica y Técnica), Ministerio de Economía y Competitividad
BIO2015-65090-R. Title: 'How does a fungal cell factory work: the different pathways of exocytosis in Aspergillus nidulans and their molecular switches'
Filamentous fungi display a remarkable capacity to secrete proteins, a capacity exploited for the production of industrial enzymes, which represents a major activity of the biotechnology sector in Europe. Yet detailed understanding of the organization and regulation of exocitosis in these organisms is wanting, including, for example, how does continuous growth by apical extension, highly demanding for exocitosis, compete with high-level secretion of enzymes. Our work on Aspergillus intracellular traffic has placed us in a privileged situation to address fundamental issues that may guide tailored improvements of productivity.