botones

V4

La unidad interdepartamental del Biotecnología Industrial (WhiteBiotech) constituye un esfuerzo conjunto de grupos activos en biotecnología microbiana y ciencia de proteínas con una trayectoria de colaboración mutua dentro del CIB. La misión de la unidad de Biotecnología Industrial es promover la excelencia en la transferencia de conocimiento y la innovación en biotecnología aplicada, actuando como un acelerador de dichas actividades a nivel nacional y europeo.

La unidad integra estrategias de investigación y tecnologías de vanguardia – incluyendo biología estructural, molecular y química, (bio)química computacional, ingeniería de proteínas, y microbiología de sistemas y sintética – para el desarrollo de nuevos productos biotecnológicos de interés para el sector de las industrias “bio-basadas” y otros, incluyendo química fina, farmacéutica y de producción a gran escala.

Nuestra conexión con estos sectores industriales tiene una dimensión internacional reconocida, tal como muestra la coordinación de dos grandes proyectos de FP7 y un proyecto-H2020 del BioBased Industries Joint-Undertaking (BBI-JU; www.bbi-europe.eu), junto con su reciente implicación en seis proyectos más de BBI-JU y H2020 (junto con varias redes ITN), y una trayectoria de proyectos relacionados financiados a nivel nacional.

 


Miembros:

WB15

La Unidad Interdepartamental fue creada en 2012 (en virtud de una decisión del CSIC de 31 de Julio) y, desde su constitución, ha experimentado varias reorganizaciones como consecuencia de cambios de los personal en el CIB y las recomendaciones del Comité Científico, quedando en la actualidad constituida por los siguientes 15 IPs: Sonsoles Martín Santamaría - Auxiliadora Prieto - Carmen Gil - Ana Martínez - Cristina Vega - Rafael Giraldo - Antonio Romero - Eduardo Díaz - Germán Rivas - María Jesús Martínez - Ángel T. Martínez (Director) - Fernando Díaz - Miguel Angel Peñalva - (sin foto: José Luis García - Ignacio Casal)

Temas de investigación de los miembros de la unidad:

1) Fabricas celulares microbianas para enzimas y metabolitos de interés, Tráfico de membrana y secreción optimizada de enzimas fúngicas,  Enzimas que actúan sobre carbohidratos (GHs, esterasas y otras), Diseño racional y evolución molecular dirigida de oxidorreductasas fúngicas,

2) Cristalografía y análisis de difracción de rayos-x, Ciencia de proteínas para el diseño de drogas, Interacciones macromoleculares y entornos celulares sintéticos

3) Genómica y metagenómica microbianas, Proteómica funcional avanzada, Biología de sistemas, Biología computacional para el diseño de enzimas y drogas

4) Biotecnología para las biorrefinerías de la lignocelulosa, Degradación/valorización microbiana (fúngica y bacteriana) de la lignina, Metabolismo microbiano de compuestos aromáticos, esteroides y otros, Bioplásticos/biomateriales a partir de recursos renovables y residuos, Microbiología sintética

 


Publicaciones seleccionadas (2014-2018):

Ayuso-Fernández I, Francisco J. Ruiz-Dueñas FJ, Martínez AT. 2018. Evolutionary convergence in lignin-degrading enzymes. Proceedings of the National Academy of Sciences of the United States of America , 201802555; DOI: 10.1073

Barriuso J, Hogan DA, Keshavarz T, Martínez MJ. 2018. Role of quorum sensing and chemical communication in fungal biotechnology and pathogenesis, FEMS Microbiology Reviews.

Barriuso J, Vaquero ME, Prieto A, Martínez MJ. 2016. Structural traits and catalytic versatility of the lipases from the Candida rugosa-like family: A review. Biotechnology Advances 34: 874-885

Carro J, Fernández-Fueyo E, Fernández-Alonso C, Cañada J, Ullrich R, Hofrichter M, … Martínez AT.(2018). Self-sustained enzymatic cascade for the production of 2,5-furandicarboxylic acid from 5-methoxymethylfurfural. Biotechnology for Biofuels, 11, 86.

Dinjaski N, Fernández-Gutiérrez M, Selvam S, Parra-Ruiz FJ, Lehman SM, Roman JS, García E, García JL, García AJ, Prieto MA. 2014. PHACOS, a functionalized bacterial polyester with bactericidal activity against methicillin-resistant Staphylococcus aureus. Biomaterials 35: 14-24

Fernández C, González-Rubio G, Langer J, Tardajos G, Liz-Marzán LM, Giraldo R*, Guerrero-Martínez A*. 2016. Nucleation of amyloid oligomers by RepA-WH1 prionoid-functionalized gold nanorods. Angewandte Chemie International Edition 55: 11237-11241

Fernández FJ, Arda A, López-Estepa M, Aranda J, Peña-Soler E, Garces F, Round A, Campos-Olivas R, Bruix M, Coll M, Tuñón I, Jiménez-Barbero J, Vega MC. 2016. Mechanism of sulfur transfer across protein-protein interfaces: The cysteine desulfurase model system. ACS Catalysis 6: 3975-3984

Fernández-Cabezón L, Galán B, García JL. (2018) Unravelling a new catabolic pathway of C-19 steroids in Mycobacterium smegmatis. Environ Microbiol 20(5):1815-1827

Hernández-González M, Bravo-Plaza I, Pinar M, de los Ríos V, Arst HN,  Peñalva MA. (2018). Endocytic recycling via the TGN underlies the polarized hyphal mode of life. PLoS Genetics, 14(4), e1007291.

Martín-Moldes Z, Blázquez B, Baraquet C, Harwood CS, Zamarro MT, Díaz E. 2016. Degradation of cyclic diguanosine monophosphate by a hybrid two-component protein protects Azoarcus sp strain CIB from toluene toxicity. Proceedings of the National Academy of Sciences of the United States of America 113: 13174-13179

Martínez AT, Ruiz-Dueñas FJ, Camarero S, Serrano A, Linde D, Lund H, Vind J, Tovborg M, Herold-Majumdar OM, Hofrichter M, Liers C, Ullrich R, Scheibner K, Sannia G, Piscitelli A, Pezzella C, Sener ME, Kilic S, van Berkel WJH, Guallar V, Lucas MF, Zuhse R, Ludwig R, Hollmann F, Fernandez-Fueyo E, Record E, Faulds CB, Tortajada M, Winckelmann I, Rasmussen JA, Gelo-Pujic M, Gutierrez A, del Rio JC, Rencoret J, Alcalde M. 2017. Oxidoreductases on their way to industrial biotransformations. Biotechnology Advances 35: 815-831

Martínez AT. 2016. How to break down crystalline cellulose. Science 352: 1050-1051

Martínez I, Mohamed MES, Rozas D, García JL, Díaz E. 2016. Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds. Metabolic Engineering 35: 46-54

Méndez-Líter JA, de Eugenio LI, Prieto A, Martínez MJ. 2018. The β-glucosidase secreted by Talaromyces amestolkiae under carbon starvation: a versatile catalyst for biofuel production from plant and algal biomass. Biotechnology for Biofuels, 11, 123.

Palomo V, Pérez DI, Roca C, Anderson C, Rodríguez-Muela N, Pérez C, Morales-García JA, Reyes JA, Campillo NE, Pérez-Castillo AM, Rubin LL, Timchenko L, Gil C, Martínez A. 2017. Subtly modulating glycogen synthase kinase 3 beta: Allosteric inhibitor development and their potential for the treatment of chronic diseases. Journal of Medicinal Chemistry 60: 4983-5001

Peláez-García A, Barderas R, Batlle R, Viñas-Castells R, Bartolomé RA, Torres S, Mendes M, López-Lucendo M, Mazzolini R, Bonilla F, de Herreros AG, Casal JI. 2015. A proteomic analysis reveals that snail regulates the expression of the nuclear orphan receptor nuclear receptor subfamily 2 group f member 6 (nr2f6) and interleukin 17 (il-17) to inhibit adipocyte differentiation. Molecular & Cellular Proteomics 14: 303-315

Pinar M, Arst HN, Pantazopoulou A, Tagua VG, de los Ríos V, Rodríguez-Salarichs J, Díaz JF, Peñalva MA. 2015. TRAPPII regulates exocytic Golgi exit by mediating nucleotide exchange on the Ypt31 ortholog RabE(RAB11). Proceedings of the National Academy of Sciences of the United States of America 112: 4346-4351

Rivas G, Minton AP. 2016. Macromolecular crowding in vitro, in vivo, and in between. Trends in Biochemical Sciences 41: 970-981

Sáez-Calvo G, Sharma A, Balaguer FA, Barasoain I, Rodríguez-Salarichs J, Olieric N, Muñoz-Hernández H, Berbís MA, Wendeborn S, Peñalva MA, Matesanz R, Canales A, Prota AE, Jiménez-Barbero J, Andreu JM, Lamberth C, Steinmetz MO, Díaz JF. 2017. Triazolopyrimidines stabilize microtubules by binding to the vinca inhibitor site of tubulin. Cell Chemical Biology 24:737-750

 


 European Projects (coordination, participation, ITNs):

EnzOx2 "New enzymatic oxidation/oxyfunctionalization technologies for added value bio-based products” (H2020 BBI-JU, 2016-2019; funding; 3.0 M€; http://enzox2.eu). The project focuses on the development of new enzymatic technologies in the production of: i) Polymer building blocks from bio-based 5-hydroxymethylfurfural; ii) Active pharmaceutical ingredients;  and iii) Terpenes as flavor & fragrance precursors. 12 partners - Coordinator Angel T. Martínez.

SusBind "Development and pilot production of sustainable bio-binder systems for wood based panels" (H2020 BBI-JU, 2018-2022; 4.4 M€ funding). The objective is to produce bio-based binders in the manufacturing of wood boards using greener technologies, such as enzymatic epoxidation. 12 partners, including world-leading companies (such as IKEA) and CIB (PI: Angel T. Martínez).

EngiCoin "Engineered microbial factories for CO2 exploitation in an integrated waste treatment platform" (H2020, 2018-2021; 7.0 M€ funding; website to be launched in Jan-2018. The project will develop new microbial factories (using Synechocystis, Ralstonia and Acetobacterium), exploiting CO2 for the production of value-added chemicals. Coordinator: Turin University (Italy). 12  partners including CIB (PI: Auxiliadora Prieto).

IBISBA 1.0 "Industrial Biotechnology Innovation and Synthetic Biology Accelerator" (H2020 INFRAIA [Integrating and opening research infrastructures of European interest]; 2018-2021; 5 M€ funding; http://www.ibisba.com). The aim is to support and accelerate the uptake of industrial biotechnology, as a key enabling technology for advanced manufacturing, by providing a network of research infrastructure facilities to promote R&D in bioprocess development. Coordinator: INRA-Toulouse (France). 16 partners including CSIC (PI: María Jesús Martínez).

WoodZymes "Extremozymes for wood based building blocks" (H2020 BBI-JU, 2018-2021; 3.3 M€ funding). The project focuses on the use of new/engineered extremophilic enzymes to substitute harsh and energy-demanding chemical treatments for sustainable production of bio-based building blocks and products in wood biorefinery. 11 partners, including CIB (Participant: Angel T. Martínez).  

LIAR "Living Architecture" (H2020, 2016-2019; 3.0 M€ funding; http://livingarchitecture-h2020.eu). LIAR is a modular bioreactor-wall, based on microbial fuel cell technology, as an integral component of human dwelling, capable of extracting valuable resources from sunlight, waste water and air, and generating oxygen, proteins and biomass. Coordinator: Newcastle University (UK). 6 partners including CIB (PI: Jose L. García).

CELBICON "Cost-effective CO2 conversion into chemicals via combination of capture, electrochemical and bi-ochemical conversion technologies" (H2020, 2016-2019; 5.4 M€ funding; http://cordis.europa.eu/project/rcn/200178_es.html). The project will bridge CO2 capture with electrochemical reduction and fermentation to bioplastics, isoprene, lactic acid and methane. Coordinator: Politecnico de Torino (Italy). 13 partners including CIB (PI: Auxiliadora Prieto).

P4SB "From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology" (FP7, 2015-2019; 7.0 M€ funding; http://www.p4sb.eu). Using Synthetic Biology to bring about the sustainable and environmentally-friendly bioconversion of oil-based plastic waste into fully biodegradable counterparts. Coordinator: RWTH Aachen (Germany). 12 partners including CIB (PI: Auxiliadora Prieto).

AFTERLIFE " Advanced filtration technologies for the recovery and later conversion of relevant fractions from wastewater" (H2020 BBI-JU, 2017-2021; 3.9 M€ funding; https://bbi-europe.eu/projects/afterlife). The project proposes a flexible, cost- and resource-efficient process for the production value-added biopolymers (polyhydroxyalkanoates) from wastewaters. Coordinator: Eggplant (Italy). 15 partners including CIB (PI: Auxiliadora Prieto).

RefuCoat "Full recyclable food package with enhanced gas barrier properties and new functionalities by the use of high performance coatings" (H2020 BBI-JU 2017-2020; 2.3 M€ funding; https://www.refucoat.eu). This project is developing hybrid bio-based high oxygen/water barrier and active coatings to be used in food packages (films and trays). Coordinator: AIMPLAS (Spain). 12 partners including CIB (PI: Auxiliadora Prieto).

PDE4NPD "Parasite-specific cyclic nucleotide phosphodiesterase inhibitors to target neglected parasitic diseases" (FP7-Health Program, 2014-2018; 6M€ funding; https://www.pde4npd.eu/). This project aims to unite global efforts to target the highly druggable class of enzymes called cyclic nucleotide phosphodiesterases. Coordinator: VU Amsterdam (Netherlands). 10 partners including CIB (PI: Carmen Gil)

LigBio "A Synthetic Biology approach for bacterial bioconversion of lignin into renewable chemicals" (Eranet-IB, 2015-2018; 0.8 M€ funding; http://www.era-ib.net/ligbio). This project will study Pseudomonas putida KT2440 to elucidate lignin degradation by bacteria, and develop a fermentation process for the production of renewable aromatics from lignin. Coordinator: Warwick University (UK). 4 partners including CIB (PI: Eduardo Diaz).

TOLLERANT "Toll-Like Receptor 4 activation and function in diseases: an integrated chemical-biology approach" (2015-2018; H2020-MSCA-ITN-2014; 3.1 M€ funding; http://www.tollerant.eu). TOLLERANT relies on synergies and combination of chemistry, biology, biophysics, biochemistry and pharmacology expertises. 7 partners including CIB (PI: Sonsoles Martin Santamaria).

DRIVE "Driving next generation autophagy researchers towards translation" (H2020 MSCA-ITN-ETN; 2017-2021, 3.8M€ funding; http://cordis.europa.eu/project/rcn/211903_en.html).  The goal is to train young scientists in the applied autophagy field. Coordinator: UMCG Groningen (Netherlands). 14 partners including CIB (PI: Ana Martinez).

V7